Kinja's being a jerk and not allowing me to post on the Open Channel, the one time I actually had non-obvious candidates in mind. Truly unfair. Especially as it's the first time Kinja actually gives me any grief. Anyway, I thought I'd share my choices here:

Gran'ma Ben from Bone. The picture speaks for itself.

Also, Grace Holloway from BioShock 2. That moment when you confront her... whoa. How could a bunch of pixels have such a presence? Also it's cool that she's not badass because of superpowers (like Gran'ma Ben's unexplained superstrength) or sci-fi technology... although she does have an army of spliced-up minions at her beck and call.